免费通用引物

引物名称	碱基数	序列
35S	20 mer	5'-GACGCACAATCCCACTATCC-3'
NOSR	18 mer	5'-AATCATCGCAAGACCGGC-3'
a-factor	21 mer	5'-TACTATTGCCAGCATTGCTGC-3'
3AOX1	21 mer	5'-GCAAATGGCATTCTGACATCC-3'
5AOX1	21 mer	5'-GACTGGTTCCAATTGACAAGC-3'
3AD	20 mer	5'-AGATGGTGCACGATGCACAG-3'
3DNA-BD	24 mer	5'-TTTTCGTTTTAAAACCTAAGAGTC-3'
3BD	25 mer	5'-TAAGAGTCACTTTAAAATTTGTATC-3'
5AD	21 mer	5'-AGGGATGTTTAATACCACTAC-3'
3GEX	23 mer	5'-CCGGGAGCTGCATGTGTCAGAGG-3'
5GEX	23 mer	5'-GGGCTGGCAAGCCACGTTTGGTG-3'
EF1a-F	21 mer	5'-TCAAGCCTCAGACAGTGGTTC-3'
PCMV-F	20 mer	5'-TCTAAAAGCTGCGGAATTGT-3'
PCMV-R	20 mer	5'-TCCAAACTCATCAATGTATC-3'
CMV-Forward	21mer	5'-CGCAAATGGGCGGTAGGCGTG-3'
CMV-R	16 mer	5'-GTTCACGGTGCCCTCC-3'
SV40-Promoter	19 mer	5'-GGAGGCTTTTTTGGAGGC -3'
EBV-Reverse	21 mer	5'-CAGCTTATAATGGTTACAAAT-3'
SV40pA-R	20 mer	5'-GAAATTTGTGATGCTATTGC-3'
BGHR	18 mer	5'-TAGAAGGCACAGTCGAGG-3'
hGH-PA-R	20 mer	5'-CCAGCTTGGTTCCCAATAGA-3'
M13(-96)	20 mer	5'-CCCTCATAGTTAGCGTAACG-3'
M13F(-47)	24 mer	5'-CGCCAGGGTTTTCCCAGTCACGAC-3'
M13F	18 mer	5'-TGTAAAACGACGGCCAGT-3'
M13R	17 mer	5'-CAGGAAACAGCTATGAC-3'
M13R(-48)	24 mer	5'-AGCGGATAACAATTTCACACAGGA-3'
pBABE-3	21 mer	5'-ACCCTAACTGACACACATTCC-3'
pBABE-5	17 mer	5'-CTTTATCCAGCCCTCAC-3'
pBAD-Forward	20 mer	5'-ATGCCATAGCATTTTTATCC-3'
pBAD-Reverse	18 mer	5'-GATTTAATCTGTATCAGG-3'
pBD-gal4-F	20 mer	5'-GCCTCTAACATTGAGACAGC-3'
pBD-gal4-R	22 mer	5'-AAGAGTTACTCAAGAACAAGAA-3'
PBV220F	20 mer	5'-AAGAAGGGCAGCATTCAAAG-3'
PBV220R	20 mer	5'-CTGCGTTCTGATTTAATCTG-3'
PTRC99C-F	18 mer	5'-TTGCGCCGACATCATAAC-3'
PEGFP-C-5	22 mer	5'-CATGGTCCTGCTGGAGTTCGTG-3'
PEGFP-C-3	20 mer	5'-TATGGCTGATTATGATCAGT-3'
PEGFP-N-5	22 mer	5'-TGGGAGGTCTATATAAGCAGAG-3'
PEGFP-N-3	22 mer	5'-CGTCGCCGTCCAGCTCGACCAG-3'
pFastBacF	20 mer	5'-GGATTATTCATACCGTCCCA-3'
pFastBacR	20 mer	5'-CAAATGTGGTATGGCTGATT-3'
p10-Promoter	19 mer	5'-CGGACCTTTAATTCAACCC-3'
pLXSN-F	20 mer	5'-CTCTCCCCCTTGAACCTCCT-3'
pLXSN-R	23 mer	5'-GAGCCTGGGGACTTTCCACACCC-3'
PMAL-C2X-F	20 mer	5'-TGCGTACTGCGGTGATCAAC-3'
PMAL-C2X-R	20 mer	5'-CTGCAAGGCGATTAAGTTGG-3'
PQE-F	20 mer	5'-TGAGCGGATAACAATTTCAC-3'
PQE-R	19 mer	5'-GTTCTGAGGTCATTACTGG-3'
RVP3	20 mer	5'-CTAGCAAAATAGGCTGTCCC-3'
RVP4	20 mer	5'-GACGATAGTCATGCCCCGCG-3'
GLP2	23 mer	5'-CTTTATGTTTTTGGCGTCTTCCA-3'
T3	20 mer	5'-ATTAACCCTCACTAAAGGGA-3'
T7	20 mer	5'-TAATACGACTCACTATAGGG-3'
T7-Term	19 mer	5'-GCTAGTTATTGCTCAGCGG-3'
pET-Up	20 mer	5'-GATCCCGCGAAATTAATACG-3'
DuetUp2	20 mer	5'-TTGTACACGGCCGCATAATC-3'
DuetDown1	20 mer	5'-GATTATGCGGCCGTGTACAA-3'
S-Tag	20 mer	5'-CGAACGCCAGCACATGGACA-3'
SP6	19 mer	5'-GATTTAGGTGACACTATAG-3'
pCold-F	19 mer	5'-ACGCCATATCGCCGAAAGG-3'
pCold-R	22 mer	5'-GGCAGGGATCTTAGATTCTGTG-3'
XL39	20 mer	5'-ATTAGGACAAGGCTGGTGGG-3'
V1_5	18 mer	5'-GGACTTTCCAAAATGTCG-3'
ITS1	19 mer	5'-TCCGTAGGTGAACCTGCGG-3'
ITS2	21 mer	5'-GCTGCGTTCTTCATCGATGC -3'
ITS3	21 mer	5'-GCATCGATGAAGAACGCAGC -3'
ITS4	20 mer	5'-TCCTCCGCTTATTGATATGC-3'
ITS5	22 mer	5'-GGAAGTAAAAGTCGTAACAAGG-3'
27F	20 mer	5'-AGAGTTTGATCCTGGCTCAG-3'
1492R	19 mer	5'-GGTTACCTTGTTACGACTT-3'
PIRES3-EGFP-R	20 mer	5'-AACGCACACCGGCCTTATTC-3'
PIRES-F	20 mer	5'-CTTACTGACATCCACTTTGC-3'
PIRES-R	20 mer	5'-CACTGCATTCTAGTTGTGGT-3'
Human-U6	18 mer	5'-GGGCAGGAAGAGGGCCTA-3'
LKO1-5	20mer	5'-GACTATCATATGCTTACCGT-3'
Mouse-U6	21mer	5'-CAGCACAAAAGGAAACTCACC-3'
pTT5-F	20mer	5'-CCGATCTGGCCATACACTTG-3'
pTT5-R	20mer	5'-TATGTCCTTCCGAGTGAGAG-3'

*您也可以下载金唯智引物与载体对照表，查看载体对应的通用引物。

金唯智通用引物选择工具

温馨提示:
使用说明:
（1）请输入您的目的载体或者需要查询的序列，点击查询，系统便会自动帮您选择测序通用引物。
（2）如果您的序列中含有数字或者空格，系统都会自动去除。