免费通用引物

            引物名称 碱基数 序列
            35S 20 mer 5'-GACGCACAATCCCACTATCC-3'
            NOSR
            18 mer 5'-AATCATCGCAAGACCGGC-3'
            a-factor
            21 mer  5'-TACTATTGCCAGCATTGCTGC-3'
            3AOX1 21 mer
            5'-GCAAATGGCATTCTGACATCC-3'
            5AOX1 21 mer
            5'-GACTGGTTCCAATTGACAAGC-3'
            3AD 20 mer
            5'-AGATGGTGCACGATGCACAG-3'
            3DNA-BD 24 mer 5'-TTTTCGTTTTAAAACCTAAGAGTC-3'
            3BD 25 mer 5'-TAAGAGTCACTTTAAAATTTGTATC-3'
            5AD 21 mer 5'-AGGGATGTTTAATACCACTAC-3'
            3GEX
            23 mer  5'-CCGGGAGCTGCATGTGTCAGAGG-3'
            5GEX 
            23 mer  5'-GGGCTGGCAAGCCACGTTTGGTG-3'
            EF1a-F
            21 mer  5'-TCAAGCCTCAGACAGTGGTTC-3'
            PCMV-F  
            20 mer 5'-TCTAAAAGCTGCGGAATTGT-3'
            PCMV-R   20 mer 5'-TCCAAACTCATCAATGTATC-3'
            CMV-Forward
            21mer 5'-CGCAAATGGGCGGTAGGCGTG-3'
            CMV-R
            16 mer 5'-GTTCACGGTGCCCTCC-3'
            SV40-Promoter 19 mer 5'-GGAGGCTTTTTTGGAGGC -3'
            EBV-Reverse 21 mer 5'-CAGCTTATAATGGTTACAAAT-3'
            SV40pA-R 20 mer 5'-GAAATTTGTGATGCTATTGC-3'
            BGHR 18 mer 5'-TAGAAGGCACAGTCGAGG-3'
            hGH-PA-R 20 mer 5'-CCAGCTTGGTTCCCAATAGA-3'
            M13(-96)
            20 mer 5'-CCCTCATAGTTAGCGTAACG-3'
            M13F(-47) 24 mer 5'-CGCCAGGGTTTTCCCAGTCACGAC-3'
            M13F 18 mer 5'-TGTAAAACGACGGCCAGT-3'
            M13R 17 mer 5'-CAGGAAACAGCTATGAC-3'
            M13R(-48) 24 mer 5'-AGCGGATAACAATTTCACACAGGA-3'
            pBABE-3 21 mer 5'-ACCCTAACTGACACACATTCC-3'
            pBABE-5 17 mer  5'-CTTTATCCAGCCCTCAC-3'
            pBAD-Forward 20 mer 5'-ATGCCATAGCATTTTTATCC-3'
            pBAD-Reverse 18 mer 5'-GATTTAATCTGTATCAGG-3'
            pBD-gal4-F 20 mer 5'-GCCTCTAACATTGAGACAGC-3'
            pBD-gal4-R  22 mer 5'-AAGAGTTACTCAAGAACAAGAA-3'
            PBV220F
            20 mer 5'-AAGAAGGGCAGCATTCAAAG-3'
            PBV220R 20 mer  5'-CTGCGTTCTGATTTAATCTG-3'
            PTRC99C-F 18 mer 5'-TTGCGCCGACATCATAAC-3'
            PEGFP-C-5  22 mer 5'-CATGGTCCTGCTGGAGTTCGTG-3'
            PEGFP-C-3 20 mer  5'-TATGGCTGATTATGATCAGT-3'
            PEGFP-N-5  22 mer  5'-TGGGAGGTCTATATAAGCAGAG-3'
            PEGFP-N-3 22 mer 5'-CGTCGCCGTCCAGCTCGACCAG-3'
            pFastBacF  20 mer  5'-GGATTATTCATACCGTCCCA-3'
            pFastBacR  20 mer 5'-CAAATGTGGTATGGCTGATT-3'
            p10-Promoter 19 mer 5'-CGGACCTTTAATTCAACCC-3'
            pLXSN-F 20 mer 5'-CTCTCCCCCTTGAACCTCCT-3'
            pLXSN-R 23 mer 5'-GAGCCTGGGGACTTTCCACACCC-3'
            PMAL-C2X-F 20 mer 5'-TGCGTACTGCGGTGATCAAC-3'
            PMAL-C2X-R 20 mer 5'-CTGCAAGGCGATTAAGTTGG-3'
            PQE-F 20 mer 5'-TGAGCGGATAACAATTTCAC-3'
            PQE-R 19 mer 5'-GTTCTGAGGTCATTACTGG-3'
            RVP3 20 mer 5'-CTAGCAAAATAGGCTGTCCC-3'
            RVP4 20 mer 5'-GACGATAGTCATGCCCCGCG-3'
            GLP2 23 mer 5'-CTTTATGTTTTTGGCGTCTTCCA-3'
            T3 20 mer 5'-ATTAACCCTCACTAAAGGGA-3'
            T7  20 mer 5'-TAATACGACTCACTATAGGG-3'
            T7-Term 19 mer  5'-GCTAGTTATTGCTCAGCGG-3'
            pET-Up  20 mer 5'-GATCCCGCGAAATTAATACG-3'
            DuetUp2 20 mer 5'-TTGTACACGGCCGCATAATC-3'
            DuetDown1 20 mer  5'-GATTATGCGGCCGTGTACAA-3'
            S-Tag  20 mer  5'-CGAACGCCAGCACATGGACA-3'
            SP6  19 mer  5'-GATTTAGGTGACACTATAG-3'
            pCold-F 19 mer  5'-ACGCCATATCGCCGAAAGG-3'
            pCold-R 22 mer  5'-GGCAGGGATCTTAGATTCTGTG-3'
            XL39
            20 mer  5'-ATTAGGACAAGGCTGGTGGG-3'
            V1_5  
            18 mer 5'-GGACTTTCCAAAATGTCG-3'
            ITS1 
            19 mer  5'-TCCGTAGGTGAACCTGCGG-3'
            ITS2 
            21 mer  5'-GCTGCGTTCTTCATCGATGC -3'
            ITS3 
            21 mer  5'-GCATCGATGAAGAACGCAGC -3'
            ITS4 
            20 mer  5'-TCCTCCGCTTATTGATATGC-3'
            ITS5
            22 mer  5'-GGAAGTAAAAGTCGTAACAAGG-3'
            27F 
            20 mer 5'-AGAGTTTGATCCTGGCTCAG-3'
            1492R 
            19 mer 5'-GGTTACCTTGTTACGACTT-3'
            PIRES3-EGFP-R 
            20 mer 5'-AACGCACACCGGCCTTATTC-3'
            PIRES-F 
            20 mer  5'-CTTACTGACATCCACTTTGC-3'
            PIRES-R  
            20 mer 5'-CACTGCATTCTAGTTGTGGT-3'
            Human-U6  18 mer  5'-GGGCAGGAAGAGGGCCTA-3'
            LKO1-5
            20mer
            5'-GACTATCATATGCTTACCGT-3'
            Mouse-U6
            21mer
            5'-CAGCACAAAAGGAAACTCACC-3'
            pTT5-F
            20mer
            5'-CCGATCTGGCCATACACTTG-3'
            pTT5-R
            20mer
            5'-TATGTCCTTCCGAGTGAGAG-3'

             

            *您也可以下载金唯智引物与载体对照表,查看载体对应的通用引物。


            金唯智通用引物选择工具

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